Journal: Journal of Clinical Virology plus

Article Title: Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

doi: 10.1016/j.jcvp.2022.100091

Figure Lengend Snippet: Comparison of detection limit of three commercial sample-to-answer platforms and the conventional real-time RT-PCR assays.

Article Snippet: In the second part of this study, we evaluated the SNP detection performance of four TIB MOLBIOL melting curve-based assays (1.

Techniques: Comparison, Quantitative RT-PCR, Variant Assay

Journal: Journal of Clinical Virology plus

Article Title: Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

doi: 10.1016/j.jcvp.2022.100091

Figure Lengend Snippet: Sensitivity and specificity of TIB MOLBIOL VirSNiP SARS-CoV-2 Spike SNP assays with reference to whole genome sequencing.

Article Snippet: In the second part of this study, we evaluated the SNP detection performance of four TIB MOLBIOL melting curve-based assays (1.

Techniques: Sequencing

Journal: Journal of Clinical Virology plus

Article Title: Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

doi: 10.1016/j.jcvp.2022.100091

Figure Lengend Snippet: Representative melting peaks of TIB MOLBIOL VirSNiP SARS-CoV-2 Spike S371L/S373P ( Fig. 1 A) and VirSNiP SARS-CoV-2 Spike E484A ( B) assays (1A) Correct melting peaks with Tm between 60-64°C were observed when Omicron specimens were tested with VirSNiP SARS-CoV-2 Spike S371L/S373P assay. (1B) Correct melting peaks with Tm >55°C were observed when Omicron specimens were tested with VirSNiP SARS-CoV-2 Spike E484A assay.

Article Snippet: In the second part of this study, we evaluated the SNP detection performance of four TIB MOLBIOL melting curve-based assays (1.

Techniques:

Journal: Journal of Clinical Virology plus

Article Title: Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays

doi: 10.1016/j.jcvp.2022.100091

Figure Lengend Snippet: Representative melting peaks of TIB MOLBIOL VirSNiP SARS-CoV-2 Spike Spike E484K ( Fig. 2 A) and VirSNiP SARS-CoV-2 Spike N501Y ( B) assays (2A) An aberrant melting peak with Tm between 42-44°C was observed when Omicron specimen was tested. The shift of melting temperature of VOC-Omicron may possibly be due to the presence of E484A mutation. (2B) An ambiguous melting peak indicated by blue arrow with Tm within 54-55°C.

Article Snippet: In the second part of this study, we evaluated the SNP detection performance of four TIB MOLBIOL melting curve-based assays (1.

Techniques: Mutagenesis

Journal: Scientific Reports

Article Title: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

doi: 10.1038/s41598-022-17339-0

Figure Lengend Snippet: Primer and probe sequences for the RT-qPCR melting curve analysis assays.

Article Snippet: In this study, we wanted to evaluate PentaBase’s RT-qPCR melting curve analysis assays for the determination of the SNPs leading to K417N/T, N439K, E484K, N501Y and P681H mutations in the S gene of SARS-CoV-2 samples by comparing these results to the reference Sanger sequencing method.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

doi: 10.1038/s41598-022-17339-0

Figure Lengend Snippet: Variant and mutation results from the RT-qPCR melting curve assays and the Sanger sequencing for the training cohort were compared how often they had similar results.

Article Snippet: In this study, we wanted to evaluate PentaBase’s RT-qPCR melting curve analysis assays for the determination of the SNPs leading to K417N/T, N439K, E484K, N501Y and P681H mutations in the S gene of SARS-CoV-2 samples by comparing these results to the reference Sanger sequencing method.

Techniques: Variant Assay, Mutagenesis, Sequencing

Journal: Scientific Reports

Article Title: Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

doi: 10.1038/s41598-022-17339-0

Figure Lengend Snippet: Variant and mutation results from the RT-qPCR melting curve assays and the Sanger sequencing for the clinical validation cohort were compared how often they had similar results.

Article Snippet: In this study, we wanted to evaluate PentaBase’s RT-qPCR melting curve analysis assays for the determination of the SNPs leading to K417N/T, N439K, E484K, N501Y and P681H mutations in the S gene of SARS-CoV-2 samples by comparing these results to the reference Sanger sequencing method.

Techniques: Variant Assay, Mutagenesis, Sequencing, Biomarker Discovery